Total phenolic content and in vitro antioxidant activity of Vigna sinensis

Rohin, Mohd Adzim Khalili and Abd Hadi , Norhayati and Mohd Yusof, Nor Farhanah and Hj. Mohamed Bassri, Siti Nafizah and Mamat, Azlina and Mat Jib @ Abd Rahman, Noor Shahhida Sazura and A. Latif, Ahmad Zubaidi (2012) Total phenolic content and in vitro antioxidant activity of Vigna sinensis. International Food Research Journal, 19 (2). pp. 409-416. ISSN 22317546

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Abstract

Vigna sinensis also known as long-podded cowpea or Chinese long bean (Family:Fabaceae) is most widely grown in Southeast Asia. They are a good source of protein, vitamin A, vitamin C, iron, phosphorus, and potassium. The antioxidant potential of crude methanol extract, chloroform, and ethyl acetate soluble fractions of Vigna sinensiswas screened for in- vitro antioxidant activity using total phenolic content, ferric reducing power, 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay, ferric thiocyanate (FTC) and thiobarbituric acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (2.69 mg/g GAE in concentration 0.5 mg/mL); more effective than methanol and chloroform extract.This fraction also exhibited fairly good antioxidant activity with in both TBA (17.39% mg/g GAE) and FTC (12.65% mg/g GAE) methods.

Item Type: Article
Keywords: Antioxidant activity, Vigna sinensis, different fractions, reductive power, phenolics content
Subjects: R Medicine > RM Therapeutics. Pharmacology
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Dr Mohd Adzim Khalili Rohin
Date Deposited: 19 May 2014 04:34
Last Modified: 28 Apr 2015 06:29
URI: http://erep.unisza.edu.my/id/eprint/915

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