Screening of Secondary Metabolites and Comparative Superoxide Radical Scavenging Along With Reducing Potency of Persea americana Using Various Solvent Extracts

Haque , Mainul and Rao , U.S. Mahadeva (2012) Screening of Secondary Metabolites and Comparative Superoxide Radical Scavenging Along With Reducing Potency of Persea americana Using Various Solvent Extracts. Drug Invention Today, 4 (11). pp. 569-574. ISSN 0975-7619

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Abstract

An effort has been made to screen the secondary metabolites, comparative superoxide radical scavenging and reducing potency of Persea americana using various solvent extracts. The fruit was extracted with various solvents. The extracts were analyzed for phytochemicals and free radical scavengers, viz. carotenoids, ascorbic acid, tocopherol, total phenol, proteins, reducing sugars and sterols. Antioxidant capacity was analyzed in terms of superoxide radial scavenging assay and reducing power assay. Screening of secondary metabolites of the different extracts revealed the presence of the phytochemicals‑alkaloids, phenols, flavonoids, sterol, saponin glycosides, reducing sugars, proteins, cardio active aglycones and cardinolides. Tremendous superoxide radical scavenging ability found in almost all extracts of P.americana. In the present study superoxide radical reduces nitro blue tetrazolium (NBT) to a blue coloured formazan that is measured at 560 nm. Free radical scavenging activity has been reported to be concomitant with development of reducing power. This shows that extracts might contain reductones like ascorbic acid, reducing sugar, thiol group containing protein which could react with free radicals to stabilize and terminate radical chain reaction. These outcomes advocate that the promising phytonutrients of the plant could be subjugated against oxidative stress, cancer, ageing, ischemic heart disease in melting thrombus, microbial infections and hormone replacement therapy (HRT) alleviating their use in traditional medicine as nutraceuticals. Key words: Persea americana, Reducing sugar, Polyphenol, Carotenoids, Ascorbic acid, Tocopherol

Item Type: Article
Keywords: Persea americana, Reducing sugar, Polyphenol, Carotenoids, Ascorbic acid, Tocopherol
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
R Medicine > RM Therapeutics. Pharmacology
Faculty / Institute: Faculty of Medicine
Depositing User: Dr U.S.Mahadeva Rao
Date Deposited: 19 May 2014 04:03
Last Modified: 28 Apr 2015 04:47
URI: http://erep.unisza.edu.my/id/eprint/898

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