PRELIMINARY STUDY ON POLYMORPHISMS OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA (PPARa) GENE IN FASTING

Mohd Rawi, Rohayu Izanwati and Mhd. Jalil, Abbe Maleyki and Ismail, Samhani and A. Latif, Ahmad Zubaidi and Zahri @ Johari, Mohd Khairi (2010) PRELIMINARY STUDY ON POLYMORPHISMS OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA (PPARa) GENE IN FASTING. In: FASTING and SUSTAINABLE HEALTH CONFERENCE 2010. Healthy Life Style Cluster, Advance Medical and Dental Institute, Universiti Sains Malaysia ( USM), Penang, Malaysia, pp. 96-100. ISBN 978-967-10331-0-4

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Abstract

Peroxisome proliferator-activated receptor (PPAR) is a nuclear receptor that functions as ligand activated transcription factors that regulate the expression by binding directly to the DNA of the target genes. PPAR binds to a DNA sequence known as a peroxisome proliferator response element (PPRE), which is located in the promoter region of the target gene. PPARa plays a crucial role in intracellular lipid metabolism. It regulates the expression of proteins involved in the transport and oxidation of free fatty acids (FFAs), predominantly in the liver. PPARa seems to have a functional role in human PBMC during fasting as several of the genes changed upon fasting were also changed upon incubation of PBMC with the specific PPARa agonist WY14, 643. However, the extent of the role of PPARa in human PBMCs remains unclear. Our aim of this study is to investigate the influence of polymorphisms of PPARa gene in mediating mechanism of enzymatic reaction during three different periods. Forty healthy volunteers were selected in order to obtain blood samples in three different conditions, pre fasting, fasting and post fasting. The samples were then stored in -80oC till the time of use. DNA from the peripheral blood of the study subjects were extracted using DNA extraction kit. The PCR methods were adapted from previous studies in order to genotype three main polymorphic sites of PPAR gene. 7.5 % substitution G to A in exon 7 was found in the sample population with no effect on amino acid threonine.The influence of the polymorphisms in PPARa gene is going to be correlated with the level of lipid profile during the three conditions afterwards.

Item Type: Book Section
Keywords: Fasting,PPARa ,Lipid profiles,Gene variant
Subjects: R Medicine > RZ Other systems of medicine
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Miss Rohayu Izanwati Mohd Rawi
Date Deposited: 26 May 2014 02:32
Last Modified: 28 Apr 2015 06:52
URI: http://erep.unisza.edu.my/id/eprint/597

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