A Comparison Between Aerial Mass Colors, Antibacterial Activities and RAPD Fingerprints of Soil Isolates of Streptomyces

Suhaili, Zarizal (2012) A Comparison Between Aerial Mass Colors, Antibacterial Activities and RAPD Fingerprints of Soil Isolates of Streptomyces. Journal of Biological Sciences, 12 (4). pp. 218-224. ISSN 1727-3048

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Abstract

This study compared the distribution of aerial mass colors, antibacterial activities and RAPD-based genomic patterns of 39 Streptomyces isolates harvested mainly from soil at the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included as a reference strain (n = 40). Based on ISP-2 media, the aerial mass colors observed were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on Mueller Hinton Agar (MHA) and Tryptic Soy Agar (TSA) by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 15 isolates with broad spectrum antibacterial activities and 18 as non-broad spectrum. TSA gave lower proportions with 15 and 9 isolates respectively. Regardless of the test media used, a higher proportion of isolates with non-white color showed antibacterial activities to suggest a potential correlation. RAPD dendrogram (a composite of 3 random primers) also clustered majority of them but segregated those of white color, which showed less antibacterial activities, in a different cluster. Further validations involving more isolates are warranted to establish the findings.

Item Type: Article
Keywords: Streptomyces, Soil, Aerial Mass, Antibacterial Activities, Random Amplified Polymorphic DNA
Subjects: Q Science > QR Microbiology
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Zarizal Suhaili
Date Deposited: 11 Jun 2014 03:07
Last Modified: 11 Jun 2014 03:07
URI: http://erep.unisza.edu.my/id/eprint/492

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