The effect of Syringin on the expression of TNF-α, iNOS, ICAM-1 and its’ mRNA in the heart, brain and kidneys of spontaneously hypertensive rats

U.S , Mahadeva Rao and Zin , Thant and C. , Shanmuga Sundaram (2016) The effect of Syringin on the expression of TNF-α, iNOS, ICAM-1 and its’ mRNA in the heart, brain and kidneys of spontaneously hypertensive rats. Der Pharmacia Lettre, 8 (3). pp. 53-61. ISSN 0975-5071

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Abstract

To investigate the effect of syringin (SYN) on TNF-α, iNOS, ICAM-1 and its’ mRNA expression in the heart, brain and kidneys of spontaneously hypertensive rats (SHR), and reveal the mechanism of its anti-inflammatory injury in hypertension target organs. SHRs were randomly divided into 5 groups including the model group (Group-2), the high-dose (G-4), medium-dose (G-5), low-dose (G-6) SYN group and the benazepril group (G-3), with 5 rats in each group. Wistar Kyoto (WKY) rats were used for the normal control group (G-1). Non-invasive blood pressure (BP) instruments were used to measure systolic blood pressure in the rats tail artery; used western blot to analyze the expression of TNF-α, iNOS, ICAM-1 and used reverse transcription-polymerase chain reaction (RT-PCR) to analyze the expression of TNF-α, iNOS, ICAM-1 mRNA. Compared with the normal control group, the model group’s BP level was significantly increased (P<0.01), but the SYN had no significant lowering effect (P>0.05); compared with the normal control group, the expression of TNF-α, iNOS, ICAM-1 and its’ mRNA in the model group was significantly increased (P<0.05 or P<0.01), and SYN could reduce the level of expression of these inflammatory cytokines (P<0.05 or P<0.01); between the benazepril group and each dose SYN group, most of the indicators had no significant difference (P>0.05). SYN had no significant BP lowering effect; SHR showed inflammatory injury in the heart, brain and kidneys; improvement on the inflammatory injury, and in turn, the anti-inflammation mechanism may be associated with lowering TNF-α, iNOS and ICAM-1 and its’ mRNA expression.

Item Type: Article
Keywords: Syringin, Spontaneously Hypertensive Rat, inflammatory factor, RT-PCR, Western Blot.
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Faculty / Institute: Faculty of Medicine
Depositing User: Dr U.S.Mahadeva Rao
Date Deposited: 10 May 2016 02:30
Last Modified: 10 May 2016 02:30
URI: http://erep.unisza.edu.my/id/eprint/4746

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