Anti-Proliferative Effect Of Duku (Lansium Domesticum Corr) Extracts on Human Colorectal Adenocarcinoma Cell Lines

Rohin, Mohd Adzim Khalili and Jumli, Mimie Noratiqah and Ridzwan, Nor Haslinda and Abd Hadi , Norhayati and Ismail, Salwani and R. Hussin, Tengku Mohammad Ariff and Wan Taib, Wan Rohani and A. Rahman, Nor Iza and A. Latif, Ahmad Zubaidi (2016) Anti-Proliferative Effect Of Duku (Lansium Domesticum Corr) Extracts on Human Colorectal Adenocarcinoma Cell Lines. Research Journal of Pharmaceutical, Biological and Chemical Sciences , 7 (2). pp. 1078-1083. ISSN 0975-8585

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Abstract

In the treatment or prevention of cancer, fruits and plants have a high content of natural antioxidants and the potential to protect humans against cancer. The duku fruit or Lansium Domesticum Corr, is rich in bioactive compounds and nutrients which important to health benefits. The purpose of this study is to screen possible anticancer activity of various extracts and fractions of duku fruit extracts that inhibit the proliferation of human colorectal adenocarcinoma (HT29). The duku fruit extract process involved three different solvents (methanol, ethanol and ethyl acetate). The human colorectal adenocarcinoma cell line was treated with concentration methanol, ethanol and ethyl acetate extracts (0-60µg/ml) from the duku fruit. The antiproliferative activities of human colon adenocarcinoma cell line treated with duku fruit extracts were determined using MTT Assays (570nm). This study found that only the methanol extract had inhibited the proliferation activity of cancer cell lines. The degree of inhibited proliferation is defined as a concentration that reduced the number of cells to 50% compared to the untreated sample (IC50). Methanol extract inhibited the proliferative HT-29 cell lines at concentration of 6.8 µg/ml. Methanol extract from the duku fruit was found to be more successful in inhibiting HT29 cell proliferation compared to the Ethanol and Ethyl Acetate extracts.

Item Type: Article
Keywords: Lansium Domesticum Corr, HT-29 Cell, Antiproliferative, MTT Assays, Methanol
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Dr Mohd Adzim Khalili Rohin
Date Deposited: 02 May 2016 07:37
Last Modified: 02 May 2016 07:37
URI: http://erep.unisza.edu.my/id/eprint/4669

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