PlasmoNexTM: a highly sensitive and specific diagnostic system for all five human Plasmodium parasites

Chew, Ching Hoong and Lee, Ping Chin and Chua, Kek Heng and Lim, Yvonne AL and Chong, Eric Tzyy Jiann and Anderios, Fread (2014) PlasmoNexTM: a highly sensitive and specific diagnostic system for all five human Plasmodium parasites. In: The 15th IUBMB 24th FAOBMB-TSBMB Conference, 21-10-2014 to 26-10-2014, Academia Sinica, Taipei, Taiwan.

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Abstract

Malaria is an infectious disease caused by Plasmodium parasite and killed more than six hundred thousand people around the world in year 2012. Malaria diagnostic techniques such as microscopy of stained blood smear requires well-trained labours yet difficulties appear to distinguish between different Plasmodium parasites whereas real-time PCR provides a fast diagnostic method but relatively expensive and might not be affordable for some laboratories. PlasmoNexTM is a new innovation to overcome these limitations, it is able to detect specific sequences in the genome of all five Plasmodium parasites in a multiplex PCR reaction with addition of an internal control to indicate the genomic DNA has been loaded into the reaction. To validate the sensitivity and specificity of the PlasmoNexTM, DNA was extracted from 207 malaria patients in Malaysia followed by Plasmodium parasite detection using PlasmoNexTM multiplex PCR and real-time PCR hydrolysis probes. The results showed that when compared to PlasmoNexTM which had a 100.00% of sensitivity and specificity in detecting Plasmodium parasites into species level, the real-time PCR only had sensitivity of 72.73% and 96.75% in detecting P. vivax and P. knowlesi species, respectively, and specificity of 98.84% in P. falciparum species detection. Besides, PlasmoNexTM is able to detect mixed Plasmodium parasite infections in a single multiplex reaction when compared to real-time PCR, indirectly saving the time and cost per sample. This study suggests that PlasmoNexTM is a highly sensitive and specific innovation that competitive to real-time PCR in human Plasmodium parasites diagnosis.

Item Type: Conference or Workshop Item (Poster)
Keywords: PlasmoNexTm, Plasmodium parasites, multiplex PCR
Subjects: Q Science > QR Microbiology
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Dr Chew Ching Hoong
Date Deposited: 09 Dec 2015 07:22
Last Modified: 09 Dec 2015 07:22
URI: http://erep.unisza.edu.my/id/eprint/4169

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