Non-toxic Antiproliferative Effect of Ficus carica Extracts on Oestrogens Receptor Breast Cancer Cell Line (MCF-7)

Umar Zubair, Rufa'i and Abu Bakar, Nor Hidayah and Gumbali Krishna Murty , Swethadri and Masaud, Idris Aliyu (2015) Non-toxic Antiproliferative Effect of Ficus carica Extracts on Oestrogens Receptor Breast Cancer Cell Line (MCF-7). In: 6th regional conference on molecular medicine in conjunction with the 2nd national conference for cancer research., 23-25 october 2015, sunway putra hotel, kuala lumpur.

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Abstract

Breast cancer is one of the commonest cancers with various therapeutic options but most of them are toxic to the body. Plants have been recognised as alternative sources of remedy for various ailments as they contain many phytochemical substances. This study was conducted to determine the total phenolic and total flavonoid contents as well as the antioxidant activity of ficus carica fruit extracts and to evaluate in-vitro anti-proliferative effect of the extract on the breast cancer cell line (MCF-7). The fruit was extracted using solvents of three different polarities. Total phenolic content was estimated by using Folin-Ciocalteu’s reagent while the total flavonoid content was estimated by using Aluminium chloride calorimetric method and both results were compared to the standard curves accordingly. Determination of the antioxidant capacity was demonstrated by 2, 2-diphenyl-1-pycrylhydrazyl (DPPH) scavenging assay. Further, the antiproliferative activity of the extracts was determined using microtitration colorimetric method of tetrazolium salt 3-(4, 5-dimethlythiazol-2-yl) 2, 5- diphenyltetrazolium bromide (MTT) reduction. Phytochemical determination revealed that ethyl acetate extract had the highest total phenolic (90.34GAE mg/g) and total flavonoid content (291.66 QE/g) in comparison with ethanol and hexane extracts. The result of the antioxidant activity also revealed the highest radical scavenging activity of the ethyl acetate extract (IC50 580 µg/ml) compared to the other extracts. The ethyl acetate extract was also found to have a strong and significant proliferative inhibitory and cytotoxic activity against the breast cancer cell line (MCF-7) with IC50 value of 9.8 µg/ml (p<0.05) whereas the hexane and ethanol extracts showed a weak inhibitory activity. Interestingly, there was no cytotoxic activity noted of the ethyl acetate extract against the normal cell line (mouse epithelial cell, (3T3)). In conclusion, this study validates the antioxidant capacity and anti-proliferative activity of the fruit of ficus carica and the positive relationship between the total phenolic and total flavonoid contents with its antioxidant activity and in-vitro anti-proliferative activity against the estrogen receptor breast cancer cell line. These may be attributed by the presence of the phenolic and flavonoids contents in the fruit extracts. The study also suggests a promising chemo-protective potential of ficus carica fruit extract on the estrogen receptor breast cancer cells.

Item Type: Conference or Workshop Item (Poster)
Subjects: R Medicine > R Medicine (General)
Faculty / Institute: Faculty of Medicine
Depositing User: Dr Nor Hidayah Abu Bakar
Date Deposited: 22 Nov 2015 02:58
Last Modified: 22 Nov 2015 02:58
URI: http://erep.unisza.edu.my/id/eprint/4036

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