CYTOTOXIC EFFECT OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE (MCF7)

Hassan, mohammad al-kassim and Azemin, Wan-Atirah and Dharmaraj, Saravanan and Mohd, Khamsah Suryati (2015) CYTOTOXIC EFFECT OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE (MCF7). Jurnal Teknologi, 77 (3). pp. 73-79. ISSN 0127–9696

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Abstract

Hepcidin (TH1-5) is a cysteine-rich antimicrobial peptide originally isolated from the freshwater fish Oreochromis mossambicus. A synthesized form of the peptide has been reported to exhibit cytotoxic activity against few human cancer cell lines. This study investigated the potential cytotoxicity of the peptide against human breast cancer cell line and normal mouse embryonic fibroblast cell line (NIH/3T3) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes by acridine orange and propidium iodide (AO/PI) double staining were also studied to determine apoptotic evidences. Hepcidin (TH1-5) showed cytotoxic activity against MCF7 with an IC50 of 20 g/mL but no significant effect against NIH/3T3. This outcome indicates hepcidin (TH1-5) to be a promising cytotoxic peptide that warrants further studies as a potential anticancer agent for breast cancer therapy

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Dr Khamsah Suryati Mohd
Date Deposited: 21 Dec 2015 02:15
Last Modified: 21 Dec 2015 02:15
URI: http://erep.unisza.edu.my/id/eprint/3984

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