Anti-Methicillin Resistant Staphylococcus aureus Activity and Optimal Culture Condition of Streptomyces sp. SUK 25

Ahmad, Siti Junaidah and Sudi, Suhaini and Mohd Sidek, Hasidah and Basri, Dayang Fredalina and Mohamad Zin, Noraziah (2015) Anti-Methicillin Resistant Staphylococcus aureus Activity and Optimal Culture Condition of Streptomyces sp. SUK 25. Jundishapur Journal of Microbiology, 8 (5). pp. 1-7. ISSN 2008-3645 (Printed) 2008-4161 (Online)

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Background: The potential of secondary metabolites extracted from Streptomyces sp. to treat bacterial infections including infections with Staphylococcus aureus is previously documented. The current study showed significant antimicrobial activities associated with endophytic Streptomyces sp. isolated from medicinal plants in Peninsular Malaysia. Objectives: The current study aimed to determine anti-methicillin-resistant-Staphylococcus aureus (MRSA) activities of Streptomyces sp. isolates. Materials and Methods: Disc diffusion and Minimum Inhibitory Concentration (MIC) assay were used to determine the antibacterial activity of Streptomyces sp. isolates. Optimization of fermentation parameters for the most potent anti-MRSA extract in terms of medium type, pH, aeration rate, and culture period was also carried out. Lastly, toxicity of the extract against Chang liver cells was determined employing the MTT, 2- (3, 5- diphenyltetrazol-2-ium-2-yl) -4, 5-dimethyl -1, 3 - thiazole; bromide assay. Results: The results indicated Streptomyces sp. SUK 25 isolates showed the most potent anti-MRSA activity. Disc diffusion assay revealed that spread plate technique was more efficient in screening anti-MRSA activity compared to pour plate (P < 0.05). To determine anti–MRSA MIC of Streptomyces sp. SUK 25, Thronton media was used. Therefore, MIC was determined as 2.44 ± 0.01 μg/mL, and accordingly, the lowest MIC was 1.95 μg/mL based on a seven-day culture, pH7, and aeration rate of 140 rpm. The crude extract was not toxic against Chang liver cells (IC50 = 43.31 ± 1.24 μg/mL). Conclusions: The Streptomyces sp. SUK 25 culturing was optimized using Thronton media, at pH 7 and aeration of 140 rpm. Further isolation and identification of bioactive compounds will develop anti-MRSA therapeutics.

Item Type: Article
Keywords: Streptomyces sp. MRSA, Culture
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Mrs Siti Junaidah Ahmad
Date Deposited: 02 Aug 2015 07:03
Last Modified: 02 Aug 2015 07:03

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