the proposed action of styrylpyrone derivative in hsv-1 infected vero cells by differential gene expression

Abd Wahab, Noor Zarina and Ibrahim, Nazlina and Abdullah Sani, Halimah and Lope Pihie, AzimahtolHawariah (2015) the proposed action of styrylpyrone derivative in hsv-1 infected vero cells by differential gene expression. Research & Reviews in Biosciences, 10 (5). pp. 166-172. ISSN 0974-7532

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Abstract

This study was done to identify and characterize specific cellular genes expression during infection of host with HSV-1 and treatment with styrylpyrone derivative (SPD). SPD showed antiviral activitywith different modes of action against HSV-1. SPD was effective in inhibiting cell death when the substance was added at 2 hours to 4 hours post infection. Cell death was only observed when treatment was delayed to 5 and 6 hours post infection. Positive effect to this mode of action suggests that SPD were able to treat HSV-1 infected cells at two effective concentration of 1.563x10-7µM and 7.813x10-8µM in treatment mode [S+V]+SPD. Differential gene expression (DEG)method was used to determine and isolate the genes that are differentially expressed inHSV-1 infectedVero cellswith and without treatment with SPD. Results from DEG analysis showed that a total of 177 genes were expressed differentially with 89 cDNAs candidates were induced and 88 cDNAs candidates were repressed. All the genes were determined by their nucleotide sequences that were compared with the database fromGenbank via bioinformatic analysis. Eightmarkers fromDEG analysis were chosen and their expressions were confirmed by using Real- Time PCR. Results from Real-Time PCR showed 100% correlation in the markers� expression profile showed by DEG method. The cDNA markers that were induced in their expression include mitogen-activated protein kinase, tapasin, carboxypeptidase M, RAB member RAS oncogen family, p53and G protein-coupled receptor.We found that SPD induced apoptosis, as measured by oncogene family gene expression and caspase activation. The apoptosis mediated by SPD in infected cells was associated with the activation of p53 and Bcl-2 protein via intrinsic pathway. SPDalso exerts its anti-HSV-1 properties by activating an extrinsic pathway via caspase-8 activation in infected cells. From this study, the understanding on how SPD acted uponHSV-1 infected host cells during the infection processwas proposed. In this study it was shown that SPD has a potential in controlling HSV-1 infection selectively without disturbing non-infected cells.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Faculty / Institute: Faculty Of Health Sciences
Depositing User: MRS NOOR ZARINA ABD WAHAB
Date Deposited: 13 May 2015 06:55
Last Modified: 13 May 2015 06:55
URI: http://erep.unisza.edu.my/id/eprint/3256

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