Gene Expressions Associated with Ultrastructural Changes in Vascular Endothelium of Atherosclerotic Lesion

Mohd Rawi, Rohayu Izanwati (2012) Gene Expressions Associated with Ultrastructural Changes in Vascular Endothelium of Atherosclerotic Lesion. World Academy of Science, Engineering and Technology, 6 (6). pp. 99-104. ISSN 1307-6892

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Attachment of the circulating monocytes to the endothelium is the earliest detectable events during formation of atherosclerosis. The adhesion molecules, chemokines and matrix proteases genes were identified to be expressed in atherogenesis. Expressions of these genes may influence structural integrity of the luminal endothelium. The aim of this study is to relate changes in the ultrastructural morphology of the aortic luminal surface and gene expressions of the endothelial surface, chemokine and MMP-12 in normal and hypercholesterolemic rabbits. Luminal endothelial surface from rabbit aortic tissue was examined by scanning electron microscopy (SEM) using low vacuum mode to ascertain ultrastructural changes in development of atherosclerotic lesion. Gene expression of adhesion molecules, MCP-1 and MMP-12 were studied by Real-time PCR. Ultrastructural observations of the aortic luminal surface exhibited changes from normal regular smooth intact endothelium to irregular luminal surface including marked globular appearance and ruptures of the membrane layer. Real-time PCR demonstrated differentially expressed of studied genes in atherosclerotic tissues. The appearance of ultrastructural changes in aortic tissue of hypercholesterolemic rabbits is suggested to have relation with underlying changes of endothelial surface molecules, chemokine and MMP-12 gene expressions.

Item Type: Article
Additional Information: International Science Index 66,2012
Keywords: Ultrastructure of luminal endothelial surface,Macrophage metalloelastase (MMP-12), Real-time PCR
Subjects: R Medicine > RZ Other systems of medicine
Faculty / Institute: Faculty Of Health Sciences
Depositing User: Miss Rohayu Izanwati Mohd Rawi
Date Deposited: 11 May 2014 09:37
Last Modified: 28 Apr 2015 04:59

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