Rhaphidophora korthalsii modulates peripheral blood natural killer cell proliferation, cytokine secretion and cytotoxicity

Yeap, Swee Keong and Omar, Abdul Rahman and Ho, Wan Yong and Beh, Boon Kee and Ali, Abdul Manaf and Banu Alitheen, Noorjahan (2013) Rhaphidophora korthalsii modulates peripheral blood natural killer cell proliferation, cytokine secretion and cytotoxicity. BMC Complementary and Alternative Medicine, 13 (2013). pp. 1-10. ISSN 1472-6882

[img] Text
00b495382fbdea58f3000000.pdf
Restricted to Registered users only

Download (746Kb) | Request a copy

Abstract

Rhaphidophora korthalsii (Araceae) is a root-climber plant which has been widely used in Chinese traditional medicine for cancer and skin disease treatment. Previous reports have recorded its immunomodulatory effects on mice splenocyte and human peripheral blood. This study investigated the potential immunostimulatory effect of Rhaphidophora korthalsii on human PBMC enriched NK cell. Methods: PBMC was exposed to various concentrations of R. korthalsii extract and the T and NK cell population in the control and extract treated PBMC were identified by immunophenotyping. Intracellular perforin and granzyme B expressions were detected by flow cytometry and extra-cellular Granzyme B, IFN-γ and TNF-α production in the isolated NK cells were determined by ELISA. The cytotoxicity of effector NK cell towards target K562 cell was assessed by CytoTox 96 assay.Rhaphidophora korthalsii methanol extract significantly increased PBMC NK cell population and intracellular perforin and granzyme B expressions. Moreover, the extract also enhanced the secretion of IFN-γ and TNF-α which subsequently enhanced the cytotoxicity of NK cell against the NK sensitive target K562 cell line. NK cell enriched with extract treated PBMC showed better activation than NK cell directly treated with the extract.Our findings indicated a potential IL-2 free immunotherapy through direct and indirect R. korthalsii stimulation on NK cell activation

Item Type: Article
Subjects: S Agriculture > SF Animal culture
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Prof Dr Abd Manaf Ali
Date Deposited: 07 Jan 2015 04:54
Last Modified: 07 Jan 2015 04:54
URI: http://erep.unisza.edu.my/id/eprint/2367

Actions (login required)

View Item View Item