Study of Cytotoxic and Antimitotic Activities of Solanum nigrum by Using Allium cepa Root Tip Assay and Cancer Chemo preventive Activity Using MCF-7- Human Mammary Gland Breast Adenocarcinoma Cell Lines

U.S , Mahadeva Rao and A., Thenmozhi and A., Nagalakshmi (2011) Study of Cytotoxic and Antimitotic Activities of Solanum nigrum by Using Allium cepa Root Tip Assay and Cancer Chemo preventive Activity Using MCF-7- Human Mammary Gland Breast Adenocarcinoma Cell Lines. International Journal of Science & Technology, 1 (2). pp. 26-48. ISSN 2250-141X

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Abstract

Objective: In the present study we have utilized the Allium cepa root tip meristem model to evaluate the cytotoxic and antimitotic activities of Solanum nigrum. The studies were extended to human cells using MCF-7- Human mammary gland breast adenocarcinoma cell lines. Methods: Preliminary antimitotic screening was done using Allium cepa root tip assay. The herbal powder obtained from plant part-dry leaves were extracted with various solvents. The antimitotic activity was analysed using Allium cepa root meristematic cells. Experiments were carried out with incorporation of folic acid in the extract. Folic acid inhibited the antimitotic activity of S.nigrum extract. Findings: The results obtained were compared with methotrexate-a known anticancer drug. Extracts of S.nigrum was found to be extremely effective in the prevention of cell proliferation of the mammary gland breast adenocarcinoma cell lines. Discussion: The pronounced antimitotic and anticancer activities of S.nigrum was due to its potential antioxidant property especially by the key role of phytochemicals such as polyphenols, steroidal saponin glycoside, alkaloids and flavonoids. Active principle sterol has been separated by TLC. Conclusion: These findings suggest that the promising antioxidant properties of the plant could be exploited in herbal preparations against oxidative stress, ageing, Ischemic heart disease in dissolving thrombus, microbial infections, hormone replacement therapy (HRT) and cancer justifying their use in traditional medicine. Keywords: Solanum nigrum; Allium cepa; Mammary carcinoma fibroblast-MCF-7 cell lines; Antimitotic

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Faculty / Institute: Faculty of Medicine
Depositing User: Dr U.S.Mahadeva Rao
Date Deposited: 08 Feb 2015 06:20
Last Modified: 28 Apr 2015 06:49
URI: http://erep.unisza.edu.my/id/eprint/2250

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