Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

Shahidan, Norshazila and Jaswir, Irwandi and Othman, Rashidi and Hs Hasyim, Yumi Zuhanis (2012) Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh. International Food Research Journal, 19 (2). pp. 531-535. ISSN 1985-4668 (Printed) 2231-7546 (Online)

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Characterization and quantification of carotenoid compound is complicated, costly and time- consuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish an analytical method for isolating ß-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of ß-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five different concentrations of ß-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year.

Item Type: Article
Keywords: Carotenoids, ß-carotene, HPLC, Cucurbita moschata
Subjects: Q Science > Q Science (General)
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Mrs Norshazila Shahidan
Date Deposited: 20 Jan 2015 08:15
Last Modified: 20 Jan 2015 08:15

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