Internal Transcribed Spacer (ITS) as a Molecular Marker for Identification of Dioscorea hispida Dennst. (Dioscoreaceae)

Samsuddin, Munirah and Mat, Nashriyah and Mazlan, Nor-Zuhailah and Omar, Siti Nor Indah and Tajuddin, Salmah and Ngah, Norhayati and Ali, Abdul Manaf (2012) Internal Transcribed Spacer (ITS) as a Molecular Marker for Identification of Dioscorea hispida Dennst. (Dioscoreaceae). Journal of Agrobiotechnology, 3. pp. 47-55. ISSN 1985-5133

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Abstract

Dioscorea hispida is a source of food and also has important medicinal values. However, there are difficulties in identifying and recognizing D. hispida based on morphological characteristics. D. hispida collected from Besut District in the state of Terengganu, Malaysia, were subjected to PCR amplification with ITS1 and ITS2 (universal primers) but no amplified product was obtained. Therefore, a new set of primers was designed based on a specific signature sequence for Dioscorea spp. in the ITS region named as Dios_003 (5’-AAA CGG CTA CCA CAT CCA AG-3’) and Dios_004 (5’-AGA CAA ATC GCT CCA CCA AC-3’). A specific band of 900 bp was detected for all accession numbers of D. hispida. Sequencing of the amplified ITS region was carried out and alignment of the sequences demonstrated that the ITS region amplified was identical in all the D. hispida accessions and was highly conserved among other Dioscorea spp. that were analyzed, i.e. D. japonica, D. alata, D. elephantipes, D. communis, D. caucasia, D. sylvatica and D. polystachya. Therefore, the new set of internal transcribed spacer (ITS) marker is an effective marker to identify D. hispida.

Item Type: Article
Keywords: Dioscorea hispida, Internal Transcribed Spacers (ITS), molecular marker
Subjects: Q Science > QK Botany
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Prof Dr Abd Manaf Ali
Date Deposited: 14 Jan 2015 06:31
Last Modified: 14 Jan 2015 06:31
URI: http://erep.unisza.edu.my/id/eprint/2078

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