Cytotoxicity, antiproliferative effects and apoptosis induction of methanolic extract of Cynometra cauliflora Linn. whole fruit on human promyelocytic leukemia HL-60 cells

Abu Bakar, Siti Aishah and TUAN JOHARI, SYED AHMAD TAJUDIN and Mat, Nashriyah and Abdul Aziz, Muhammad Yusran and Alwi @ Ali, Afnani and Ali, Abdul Manaf (2012) Cytotoxicity, antiproliferative effects and apoptosis induction of methanolic extract of Cynometra cauliflora Linn. whole fruit on human promyelocytic leukemia HL-60 cells. Evidence-based Complementary and Alternative Medicine, 2012. ISSN 1741-427X (Printed) 1741-4288 (Online)

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230847062_Title_Cytotoxicity_antiproliferative_effects_and_apoptosis_induction_of_methanolic_extract_of_Cynometra_cauliflora_Linn._whole_fruit_on_human_promyelocytic_leukemia_HL-60_cells - Published Version
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Abstract

Abstract Methanolic extract of Cynometra cauliflora whole fruit was assayed for cytotoxicity against the human promyelocytic leukemia HL-60 and the normal mouse fibroblast NIH/3T3 cell lines by using the microtetrazolium (MTT) assay. The cytotoxic dose fifty percent (CD50) of the extract, the concentration that reduced 50% of the HL-60 cells treated for 72 hours was 0.9 µg/mL whereas the value for the cytotoxic drug vincristine was 0.2 µg/mL. The extract was found to be less cytotoxic towards the NIH/3T3 cells, where the viability of the cells was at 80.0% when treated at 15.0 µg/mL. The extract was inhibited HL-60 cell proliferation with dose dependent. Acridine orange/Iodine (AO/PI) staining of HL-60 cells treated with the extract for 72 hours revealed that majority of cells were in the apoptotic cell death mode. Flow cytometry analysis of HL-60 cells treated at CD50 the extract and stained with annexin-V/propidium iodide, showed the early apoptotic cells were 31.0, 26.3 and 19.9% at 24, 48 and 72 hours treatment, respectively. The percentage of late apoptotic cells were increased from 62.0 at 24 hour to 64.1 and 70.2 at 48 and 72 hours, respectively. Meanwhile, the percent of necrotic cells were 4.9, 6.6 and 8.5 at 24, 48 and 72 hours, respectively. This study has shown that the methanolic extract of C. cauliflora whole fruit was cytotoxic towards HL60 cells and induced the cells into apoptotic cell death mode but less cytotoxic towards NIH/3T3 cells. Keywords: Cynometra cauliflora, apoptosis, antiproliferative, HL-60 cells, NIH/3T3 cells

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SF Animal culture
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Siti Aishah Abu Bakar
Date Deposited: 14 Jan 2015 04:50
Last Modified: 14 Jan 2015 04:50
URI: http://erep.unisza.edu.my/id/eprint/1894

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