Molecular Characterization of Anopheles Malariosis in Area of Pakistan.: a study done in Islamabad and Rawalpindi

Noreen , Moin and Atif, A Baig (2012) Molecular Characterization of Anopheles Malariosis in Area of Pakistan.: a study done in Islamabad and Rawalpindi. 1, 1 (1). Creatspace, USA. ISBN 978-1477547618

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Malaria is an internationally destructive infectious disease, by which, one million people die every year. It is reported to affect about 500,000 people in Pakistan per year. Malaria is caused by protozoan parasites of the plasmodium genus. The mosquito specie which acts as vector belongs to genus Anopheles. The reported species in Pakistan are Anopheles stephensi and Anopheles culicifacies. The found vector in study area was Anopheles stephensi only because of the water logging of soil and salinization has created an environment which is not favorable for A. culicifacies breeding while such environment favors more salt-tolerant and less efficient vector A. stephensi. The identification of vector specie is really very important for the development of any control strategies. It is difficult to identify the members of the same specie accurately when some members act as vector while other are non vectors, so the use of DNA markers instead of morphological marker is best approach. The present study was designed to identify the vector of malariosis by using DNA markers instead of morphological markers, the used markers were, one for COI of mtDNA and other one was for ITS2 of rDNA. DNA was extracted from single mosquitoes by using Qiagen Tissue Kit (Qiagen, Hilden, Germany) and by following the manufacturer’s protocol with some modifications. Two used primers i.e. COI-F/R and 5.8s-F and 28s-R, showed DNA amplified for the target sequences in PCR. After the procedure of gel electrophoresis, the amplified products were analyzed after staining and visualizing in UV transilluminator. Appearance of band 682 bp with COI-F/R primers and 650 bp with 5.8s-F and 28s-R on gel indicated the amplification of A. stephensi DNA leading to the confirmation of specie. Thus, the results of investigations provide us with sufficient data for the development of taxonomic markers in order to differentiate among existing A. stephensi species. This novel data could also be used to sequence the amplified regions of mtDNA and rDNA of A. stephensi.

Item Type: Book
Subjects: R Medicine > R Medicine (General)
Faculty / Institute: Faculty of Medicine
Depositing User: Dr Atif Baig
Date Deposited: 20 Jan 2015 03:24
Last Modified: 28 Apr 2015 06:25

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