Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii

Nakisah, M. A and Ida Muryany, M. Y and Fatimah, H and Nor Fadilah, R and Zalilawati, M. R and Mohd, Khamsah Suryati and Habsah, M (2012) Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii. World Journal of Microbiology and Biotechnology, 28 (3). pp. 1237-1244. ISSN 0959-3993

[img] Text
Nakisah et al 2011.pdf
Restricted to Registered users only

Download (444Kb)
Official URL: http://www.springer.com/journal/11274

Abstract

Crude methanol extracts of a marine sponge, Aaptos aaptos, collected from three different localities namely Kapas, Perhentian and Redang Islands, Terengganu, Malaysia, were tested in vitro on a pathogenic Acantha- moeba castellanii (IMR isolate) to examine their anti- amoebic potential. The examination of anti-Acanthamoebic activity of the extracts was conducted in 24 well plates for 72 h at 30 °C. All extracts possessed anti-amoebic activity with their IC50 values ranging from 0.615 to 0.876 mg/mL. The effect of the methanol extracts on the surface morphol- ogy of A. castellanii was analysed under scanning electron microscopy. The ability of the extracts to disrupt the amoeba cell membrane was indicated by extensive cell’s blebbing, changes in the surface morphology, reduced in cell size and with cystic appearance of extract-treated Acanthamoeba. Number of acanthapodia and food cup was also reduced in this Acanthamoeba. Morphological criteria of apoptosis in Acanthamoeba following treatment with the sponge’s extracts was determined by acridine orange-propidiumiodide staining and observed by fluorescence microscopy. By this technique, apoptotic and necrotic cells can be visualized and quantified. The genotoxic potential of the methanol extracts was performed by the alkaline comet assay. All methanol extracts used were significantly induced DNA damage compared to untreated Acanthamoeba by having high percentage of scores 1, 2, and 3 of the DNA damage. Results from cytotoxicity and genotoxicity studies carried out in the present study suggest that all methanol extracts of A. aaptos have anti-amoebic properties against A. castellanii.

Item Type: Article
Subjects: Q Science > QR Microbiology
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Dr Khamsah Suryati Mohd
Date Deposited: 13 Jan 2015 08:44
Last Modified: 13 Jan 2015 08:44
URI: http://erep.unisza.edu.my/id/eprint/1645

Actions (login required)

View Item View Item