Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Okuma, Eiji and Jahan, Md Sarwar and Munemasa, Shintaro and Hossain, Mohammad Anowar and Muroyama, Daichi and Islam, Mohammad Mahbub and Ogawa, Ken'ichi and Watanabe-Sugimoto, Megumi and Nakamura, Yoshimasa and Shimoishi, Yasuaki and Mori, Izumi C and Murata, Yoshiyuki (2011) Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis. Journal of Plant Physiology, 168 (17). pp. 2048-55. ISSN 1618-1328

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Abstract

We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca(2+)-permeable channel currents by ABA or oscillation of the cytosolic free Ca(2+) concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca(2+) oscillation in ABA signal pathway of Arabidopsis guard cells.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Dr. Md Sarwar Jahan
Date Deposited: 21 Jan 2015 08:23
Last Modified: 21 Jan 2015 08:23
URI: http://erep.unisza.edu.my/id/eprint/1629

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