Effects of Different Stages of Cryopreservation of Red Tilapia (Oreochromis niloticus) Sperm and the Variability between Three Individual Fish in Response to Cryopreservation

Kari, Asmad and Wan Embong, Wan Khadijah and Abdullah, Ramli (2011) Effects of Different Stages of Cryopreservation of Red Tilapia (Oreochromis niloticus) Sperm and the Variability between Three Individual Fish in Response to Cryopreservation. Journal of Agrobiotechnology, 2. pp. 25-33. ISSN 1985-5133

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Abstract

Cryopreservation is a technique that makes long term storage of fish semen possible. The objectives of the present study were to determine the effects of different stages of cryopreservation and individual variability of fish in the cryopreservation process. This study involved five stages of cryopreservation (fresh sperm, sperm + extender, after equilibration time, after vapor phase and after freezing) and three different fish. Sperm movement and velocity distribution for fresh and after frozen-thawed semen were evaluated using an automated semen analyzer. The motility of red tilapia fish sperm decreased gradually in the freezing process. The highest value of motility was obtained at fresh sperm (96.93 0.53%), followed by sperm with extender (92.64 1.05%), after equilibration time (84.86 2.26%) and after vapor phase (67.82 3.28%), whilst the lowest value of motility was obtained for sperm after freezing (61.39 3.62%). For individual fish, Fish 1 significantly showed the highest total motility (77.50 1.86%). Keywords: Cryopreservation, red tilapia sperm, sperm motility

Item Type: Article
Subjects: S Agriculture > SF Animal culture
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Dr Asmad Kari
Date Deposited: 04 Feb 2015 07:40
Last Modified: 04 Feb 2015 07:40
URI: http://erep.unisza.edu.my/id/eprint/1616

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