Histochemical quantification of glutathione contents in guard cells of Arabidopsis thaliana

Jahan , Md Sarwar and Nakamura, Yoshimasa and Murata, Yoshiyuki (2011) Histochemical quantification of glutathione contents in guard cells of Arabidopsis thaliana. ScienceAsia, 37. pp. 281-284. ISSN 1513-1874

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Glutathione (GSH), one of the most abundant low molecular-weight thiol compounds maintains redox homeostasis in plants under normal and stressful conditions. In order to measure GSH content in guard cells of Arabidopsis thaliana, we have developed a technique to quantify the GSH content in guard cells using GSH staining dye, monochlorobimane (MCB). The fluorescent intensity of glutathione S-bimane (GSB) gradually increased in guard cells with increasing incubation time and reached steady state 2 h after epidermal tissues were treated with MCB. The guard cells contained larger amount of GSH content than other cells of leaves except trichome cell. MCB staining showed that guard cells of ch1-1, a GSH deficient mutant, accumulated significantly lower GSH content than that of wild type guard cells. When the guard cells were treated with 1-chloro-2,4-dinitrobenzene (CDNB) to reduce GSH content in the guard cells, the GSB fluorescence in the guard cells was abolished. In addition, GSH monoethyl ester (GSHmee) restored GSH level in the guard cells of ch1-1 mutant plants. These results suggest that GSH content in guard cells can be quantified using MCB dye.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Faculty / Institute: Faculty of Bioresources & Food Industry
Depositing User: Dr. Md Sarwar Jahan
Date Deposited: 21 Jan 2015 08:17
Last Modified: 21 Jan 2015 08:17
URI: http://erep.unisza.edu.my/id/eprint/1614

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